Figure 2.

Transcriptome analysis of DEGs in fibers of GhWRKY16-RNAi transgenic cotton. A, Volcano of DEGs between the wild-type and GhWRKY16-RNAi lines. The red dots represent upregulated genes and the blue dots represent downregulated genes in 9 DPA fibers of the GhWRKY16-RNAi lines. B, Major classes of downregulated genes in GhWRKY16-RNAi fibers. C, RT-qPCR analysis of genes related to cotton fiber development in 9 DPA fibers of GhWRKY16 RNAi lines. Total RNA was isolated from 9 DPA fibers of the GhWRKY16 RNAi lines and wild-type controls. GhUBI1 (EU604080) was used as internal reference. Error bars represent sd of three biological replicates. *P <0.05; **P <0.01 by independent t-tests. WT, wild-type; L1, L3, and L5, GhWRKY16-RNAi cotton lines