FIG. 4.

Induction of the endogenous IRF-1 gene by Stat1-ER in response to estrogen. Cos7 cells were transfected with an expression vector for Stat1-ER and treated for the indicated time with estrogen. Total RNA was prepared and subjected to RT-PCR analysis using specific primers for the IRF-1 gene. The PCR products were analyzed by PAGE, exposed to film (shown), and quantified by PhosphorImager analysis to give the fold inductions indicated (after normalization from parallel analyses of the housekeeping gene GAPDH).