Figure 2.

miR-586 induced Wnt/β-catenin signaling activation in breast cancer

(A) Luciferase analysis of TGF-β, NF-κB, Wnt, Notch, and Hedgehog signaling activity in the indicated cells. (B) Effect of miR-586 on the nuclear localization of β-catenin in the indicated cells, as determined by immunofluorescence staining. (C) Subcellular fractionation and subsequent western blot assays were performed to reveal the effect of miR-586 on the subcellular distribution of β-catenin in the indicated cells. (D) Immunohistochemical staining analysis expression level of β-catenin in breast cancer tissues with high or low miR-586 expression. The correlation between expression of miR-586 and nuclear localization shown with percentage of specimens. (E) The effect of miR-586 on the interactions of β-catenin with TCF4 in the indicated cells, as determined by IP. ∗p < 0.05.