Figure 8. In vivo basal PIP₂ levels are decreased in microglia of Plcg2 ^R522 mice compared to Plcg2 ^P522 mice.

• A–C
  Iba1 was used as a marker for microglia, and PIP₂ levels were detected by measuring the intensity of florescence per cell. The average fluorescence was calculated above background in the cortex and hippocampus at 2 (A), 6 (B) and 9 (C) months. All data show the mean ± SD of three independent experiments and were analysed using two‐way ANOVA with Sidak’s multiple comparison tests performed. **P < 0.01 and ****P < 0.0001. See also Appendix Fig S7.