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. 2021 May 25;40(17):e106887. doi: 10.15252/embj.2020106887

Figure 4. Ntc linkers give high flexibility and elasticity to S‐Enas.

Figure 4

  • A
    CryoEM image of an isolated ex vivo S‐Ena making a U‐turn comprising 19 helical turns (shown schematically in orange), i.e., corresponding to a ˜10° axial tilt between consecutive helical turns.
  • B, C
    Cross‐section of the molecular surface of the ex vivo S‐Ena model and the ex vivo S‐Ena 3D cryoEM electron potential map. Model and map reveal a 4.5 Å longitudinal spacing between Ena1B jellyroll domains (red dashed lines) as a result of the 10 Å long Ntc linker (residues 12–17).
  • D
    Negative stain 2D class averages of endospore‐associated S‐Enas show variation in pitch and axial curvature, likely as a result of strain exerted during deposition of spores on the grid surface. The observed range in pitch corresponds to a range in longitudinal spacing of 2.5 to 12 Å between helical turns (labeled red in panel B).