Figure 7. Ena is widespread in pathogenic Bacilli.

• A
  Ena1 and Ena2 loci with average amino acid sequence identity indicated between the population of EnaA‐C ortho‐ and homologs. Ena1C shows considerably more variation and is in B. cytotoxicus different from both Ena1C and Ena2C (see Fig EV5), while other genomes have enaC present at different loci (applies to two isolates of B. mycoides).
• B
  Distribution of ena1/2A‐C among Bacillus species. Whole genome clustering of the B. cereus s.l. group and B. subtilis created by Mashtree (Katz et al, 2019; Ondov et al, 2016) and visualized in Microreact (Argimon et al, 2016). Rooted on B. subtilis. Traits for species (colored nodes), Bazinet clades and presence of ena are indicated on surrounding four rings in the following order from inner to outer: clade according to Bazinet, 2017 (when available) (Bazinet, 2017), and presence of enaA, enaB, and enaC, resp. (for all three, ena1: teal, ena2: orange, different locus: cyan). When no homo‐ or ortholog was found, the ring is gray. Interactive tree accessible at: https://microreact.org/project/vn2oWw7zM3cwejEFNoRGWA/0024f86c.