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. 2021 Jul 29;40(17):e108588. doi: 10.15252/embj.2021108588

Figure 3. EDNA detects intracellular neutralization activity in SARS‐CoV‐2 convalescent sera.

Figure 3

  • A
    Capillary‐based protein detection (Jess) of antibodies against SARS‐CoV‐2 antigens in convalescent sera.
  • B
    Quantification of antigen‐specific antibodies in convalescent sera.
  • C
    293T cells OE ACE2 infected with SARS‐CoV‐2 in the presence of electroporated convalescent sera. There are statistically significant differences in the ability of serum from different individuals to inhibit viral replication intracellularly (**P < 0.005, *P < 0.05).
  • D
    293T ACE2 OE/TRIM21 KOs reconstituted with either empty vector (EV) or TRIM21‐expressing vector (TRIM21) infected with SARS‐CoV‐2 in the presence of electroporated convalescent sera. Intracellular neutralization of viral replication is only observed in cells reconstituted with TRIM21 (**P < 0.005).

Data information: All data represent at least three independent replicates. Error bars depict the mean ± SEM. Statistical comparisons were performed using a one‐way (C) or two‐way (D) ANOVA.