Figure 4. SARS‐CoV‐2 convalescents make potently neutralizing N antibodies whose activity correlates with the number of active N‐specific T cells.

• A
  Capillary‐based protein detection (Jess) of N antibodies in convalescent sera, quantified by chemiluminescence.
• B
  Quantification of SARS‐CoV‐2 intracellular neutralization by electroporated convalescent sera using EDNA and RT–qPCR (as % of PBS condition).
• C
  Correlation between N‐antibody titre, as calculated by Jess, and intracellular neutralization (fold decrease in SARS‐CoV‐2 replication), as measured by EDNA, in convalescent sera from multiple individuals.
• D
  Intracellular neutralization of SARS‐CoV‐2 by titrated serum from two strongly N antibody‐positive individuals.
• E
  ELISpot measuring IFNγ production in T cells from SARS‐CoV‐2 convalescents upon stimulation with a peptide library covering N‐protein.
• F
  Correlation between N‐specific T‐cell activity and intracellular neutralization activity.
• G
  Correlation between N‐specific T‐cell activity and N‐antibody titre.

Data information: All data represent at least three independent replicates. Error bars depict the mean ± SEM. Statistical analysis was performed using a semilog non‐linear fit (F, G).