Fig. 1.

Expression of myelin proteins in the postnatal Cp^KOmouse. (A) P2 Sox10-Cp^KO, NG2-Cp^KO and control (Cre-negative) littermates received 5 consecutive tamoxifen injections and brain tissue was collected at P15 and P30. (B) Semi-quantitative RT-PCR for Cp was performed at P15 with RNA isolated from the corpus callosum of control and Sox10-Cp^KO mice. (C) PLP and MBP immunostaining in the brain of control and Sox10-Cp^KO mice at P15 and P30. Scale bar = 180 μm. (D and E) PLP and MBP expression was quantified in Sox10-Cp^KO and NG2-Cp^KO brains by analyzing the integrated fluorescence intensity in the somatosensory cortex (CX) and central corpus callosum (CC). Values are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. respective controls.