Fig. 6.

Myelin synthesis in the aged Cp^KOCNS. (A) 8-month-old Sox10-Cp^KO and control (Cre-negative) littermates were injected with tamoxifen every other day during two weeks, and brain tissue was collected at 10 and 12 months (10M and 12M). (B) PLP and MBP immunostaining in the brain of control and Sox10-Cp^KO mice at 12 M. Scale bar = 180 μm. (C) PLP and MBP expression was quantified by analyzing the integrated fluorescence intensity in the somatosensory cortex (CX), striatum (ST) and central and lateral corpus callosum (CCC and LCC, respectively). Values are expressed as mean ± SEM. ***p < 0.001 vs. respective controls. (D) Electron micrographs of axons in the corpus callosum of control and Sox10-Cp^KO mice at 12 M. Scale bar = 8 μm upper panel; 2 μm lower panel. (E) Mean g-ratio values and percentage of myelinated axons at 10 M and 12 M. (F) Scatter plot of g-ratio values of myelinated axons. Values are expressed as mean ± SEM. **p < 0.01, ***p < 0.001 versus control. (G) Western blots for MBP and PLP are shown. P84 was used as the internal standard and box-and-whisker plots are showing means ± SD. *p < 0.05, **p < 0.01 vs. respective controls.