Fig. 9.

Neuronal densities and oxidative stress in Cp^KObrains. (A and D) Representative coronal sections from control and Sox10-Cp^KO brains immunostained for NeuN, Olig2 and 8-OHdG at 12 M. (A) scale bar = 180 μm; (D) scale bar = 180 μm upper panel; 90 μm lower panel. (B) NeuN-positive cells, cortical thickness and neurofilament L fluorescence intensity were quantified in the somatosensory cortex at P30, P90 and 12 M. (C) Representative western blots for NeuN and neurofilament M at 12 M. P84 was used as the internal standard and box-and-whisker plots are showing means ± SD. **p < 0.01 vs. respective controls. (E) 8-OHdG fluorescence intensity and Olig2/8-OHdG and NeuN/8-OhdG double-positive cells were quantified in the somatosensory cortex at P30, P90 and 12 M. Scatter dot-plot graphs are showing single brain slices values plus mean ± SD. ***p < 0.001 vs. respective controls. (F) Semi-quantitative RT-PCRs for superoxide dismutase (SOD), glutathione peroxidase (GPX) and heme oxygenase-1 (HO-1) were performed at 12 M with RNA isolated from the cortex of control and Sox10-Cp^KO mice. GAPDH was used as the internal standard and data are summarized based on the relative spot intensities and plotted as percent of controls. Box-and-whisker plots are showing means ± SD. ***p < 0.001 vs. respective controls.