Fig. 1.

Timeline for studying the effect of radiation and metformin-induced neuroanatomical changes. Approximate human-equivalent ages are depicted above the timeline. Mice were irradiated at P16. Metformin MET16 or MET19 was administered for 14 days, starting on P16 or P19, respectively. In vivo MRI was performed on P14, P23, P42, and P98. BrdU was administered for 7 days, starting on P28, and mice were perfused on P42 in preparation for immunohistochemistry (IHC). Abbreviations: MET16, treated with metformin starting at P16; MET19, treated with metformin starting at P19; MRI, magnetic resonance imaging.