Skip to main content
. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Respir Physiol Neurobiol. 2019 Jul 25;269:103250. doi: 10.1016/j.resp.2019.103250

Figure 1. A. Stimulating electrode placement and localized region of nTS patched. B-D. Mean passive membrane properties of 2nd-order nTS neurons 7 to 10 days after intratracheal installation of bleomycin.

Figure 1.

Data presented in this figure was collected from whole cell patched 2nd-order nTS neurons. A. Representative photo of the 300 μM horizontal slice with the nylon harp placed on the outer edge. The stimulating electrode was gently positioned upon the darkly striated tractus solitarii distal to the site of recording. The caudal area of the medial nTS can be seen at the tip of the recording pipette, and was visualized with DIC at 5x, and a whole cell patch was made. B-D. Membrane resistance (B), membrane capacitance (C) and resting membrane potential (RMP) (D) were recorded in Bleo (n=12 neurons, 12 slices, 12 rats) and saline (n=14 neurons, 14 slices, 14 rats) treated rats to determine whether acute lung injury altered the passive membrane properties of 2nd-order nTS neurons. Mean data shows that 7–10 days following bleomycin-induced lung injury there was no significant difference in the membrane resistance (P=0.10), membrane capacitance (P=0.55) or resting membrane potential (P=0.34) between saline and Bleo groups. Data is represented as mean ± standard deviation.