Figure 2. Amplitude is significantly decreased, and rise and decay times are significantly slower in spontaneous EPSCs following acute lung injury.

Data presented in this figure was collected from whole cell patched 2^nd-order nTS neurons. A. Representative trace from saline and Bleo treated 2^nd-order nTS neurons showing sEPSCs over the course of a 30 second epoch. B-E. Cumulative probability plots with inset bar graphs representing mean data ± standard deviation for sEPSC amplitude (B), rise time (C), decay time (D), and interevent interval (frequency) (E) in 2^nd-order nTS neurons from Bleo and saline groups. There was a significant decrease (P=0.04) in sEPSC amplitude (B) between Bleo and saline neurons that was reflected in a leftward shift in the cumulative probability plot. There was also a significant increase (P<0.01) in sEPSC rise time (C) that was reflected in a rightward shift in the cumulative probability plot, and a significant increase (P=0.03) in sEPSC decay time (D) that was reflected in a rightward shift of the cumulative probability plot. The frequency (E) of spontaneous sEPSCs was not significantly different (P=0.39) between Bleo and saline groups. Bleo group (n=12 neurons, 12 slices, 12 rats); saline group (n=14 neurons, 14 slices, 14 rats). Cumulative probability plots represent all cells patched from Bleo and saline treated rats. F. Representative trace overlaying a single sEPSC from a saline and Bleo 2^nd-order nTS neuron.