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. Author manuscript; available in PMC: 2022 Aug 24.
Published in final edited form as: Biochemistry. 2021 Aug 6;60(33):2524–2536. doi: 10.1021/acs.biochem.1c00384

Table 2.

Steady-state kinetic parameters for derivative substrates compared to FRKacRW

Substrate KM
(μM)
kcat
(s−1)
kcat/KM
(M−1 s−1)
FRKacRW 500 ± 50 0.233 ± 0.006 470 ± 40
FAKacRW 3100 ± 1400 0.15 ± 0.04 48 ± 25
FRKacAW 680 ± 160 0.43 ± 0.04 630 ± 160

Statistically significant difference from the value for FRKacRW.