Fig. 4.

Correlation between reversion of cell growth and remodeling of microtubule cytoskeleton. A Ovarian cancer cells (OVCAR5, OVCAR4, OVCAR8, OVCAR10, and A2780) were treated with or without paclitaxel (1 nM, Taxol), and in 8 h, the cells on tissue culture dish were exposed to ultrasound (+ US, 40 KHz, 2.5 W/square inch) for 5 min. B Cell number was determined by WST-1 assay on Day 3. The differences in cell numbers between PTX and PTX + US are statistically significant determined by Student-T test: p = 0.01 and 0.008 for A2780 and OVCAR5 respectively. C After recovery from ultrasound exposure for 2 h, the cells were analyzed by immunofluorescence microscopy for alpha tubulins. A representative example of OVCAR5 cells is shown