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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2019 Dec 30;389:114876. doi: 10.1016/j.taap.2019.114876

Table 1:

List of fluorescent labels used in the present study.

Assay Organelle / purpose Label Live-label? Excitation / Emission wavelength1 [nm] Provider Cat-No Stock concentration In well concentra tion
CV Nucleus Hoechst-33342 Y 405 / 435–480 Invitrogen H3570 16.2 mM in H2O 7.7 μM
Cytotoxicity PI Y 561 / 570–630 Invitrogen P3566 1.5 mM in H2O 3.6 μM
CP Nucleus Hoechst-33342 N 405 / 435–480 Invitrogen H3570 16.2 mM in H2O 6.75 μM
Nucleoli / RNA SYTO14 N 488 / 500–550 Invitrogen S7576 5 mM in DMSO 3 μM
ER Concanavalin A-488 N Invitrogen C11252 1 mg/ml in 0.1 M NaHCO3 pH 8.37 with 2 mM NaN3 100 μg/ml
Actin skeleton Alexa Fluor 568 Phalloidin N 561 / 570–630 Invitrogen A12380 6.6 μM in methanol 41.2 nM
Golgi + plasma membrane Wheat Germ Agglutinin, Alexa Fluor 555 Conjugate N Invitrogen W32464 1 mg/ml in PBS 5 μg/ml
Mitochondria MitoTracker DeepRed Y 640 / 650–760 Invitrogen M22426 1 mM in DMSO 475 nM
1

Excitation / emission wavelengths refer to the Opera Phenix microscope.