Table 1:
List of fluorescent labels used in the present study.
Assay | Organelle / purpose | Label | Live-label? | Excitation / Emission wavelength1 [nm] | Provider | Cat-No | Stock concentration | In well concentra tion |
---|---|---|---|---|---|---|---|---|
CV | Nucleus | Hoechst-33342 | Y | 405 / 435–480 | Invitrogen | H3570 | 16.2 mM in H2O | 7.7 μM |
Cytotoxicity | PI | Y | 561 / 570–630 | Invitrogen | P3566 | 1.5 mM in H2O | 3.6 μM | |
CP | Nucleus | Hoechst-33342 | N | 405 / 435–480 | Invitrogen | H3570 | 16.2 mM in H2O | 6.75 μM |
Nucleoli / RNA | SYTO14 | N | 488 / 500–550 | Invitrogen | S7576 | 5 mM in DMSO | 3 μM | |
ER | Concanavalin A-488 | N | Invitrogen | C11252 | 1 mg/ml in 0.1 M NaHCO3 pH 8.37 with 2 mM NaN3 | 100 μg/ml | ||
Actin skeleton | Alexa Fluor™ 568 Phalloidin | N | 561 / 570–630 | Invitrogen | A12380 | 6.6 μM in methanol | 41.2 nM | |
Golgi + plasma membrane | Wheat Germ Agglutinin, Alexa Fluor™ 555 Conjugate | N | Invitrogen | W32464 | 1 mg/ml in PBS | 5 μg/ml | ||
Mitochondria | MitoTracker DeepRed | Y | 640 / 650–760 | Invitrogen | M22426 | 1 mM in DMSO | 475 nM |
Excitation / emission wavelengths refer to the Opera Phenix microscope.