FIGURE 2.

ZIPK reduced the antitumor activity of cisplatin in gastric cancer cells. (A) GC cells were treated with cisplatin at different concentrations for 24 h and cell survival was measured by CCK‐8 assay. Three independent assays were conducted. The results were expressed as the mean ± SD (*, P < 0.05; **, P < 0.01; ***, P < 0.001, Student’s t‐test). (B) The evaluation of IC50 to cisplatin in HGC27 and SGC7901 cells after 24 h. (C) Cell viability was determined by colony formation assay after cisplatin treatment for 2 weeks. (D) Cell death was determined by trypan blue staining assay after 24‐h treatment with cisplatin (1 μg·mL⁻¹). (E) qPCR analysis of gene expression in ZIPK‐overexpressed, ZIPK‐silenced, and their respective control cells. Three independent assays were performed. The results were expressed as the mean ± SD (*, P < 0.05; **, P < 0.01; ***, P < 0.001, Student’s t‐test)