Fig. 3.

Effects of the additions of FBS/BSA in collecting buffer on cell counts. (A) Jurkat cells. (B) 293T cells. (C) CD45⁺ cells from mouse spleen. Cell counts for sorted cells using different collecting buffers. We collected 500 000 cells using each collecting buffer. A 50 μL cell suspension was tested by flow cytometry. The actual cell amounts were counted. Based on the total cell amounts and weight difference before and after sorting to estimate the theoretical cell amounts in 50 μL collecting buffer, the recovery rate was obtained by the actual cell amounts/the theoretical cell amounts. Data are presented as the mean ± SEM. n = 3. Each experiment was repeated at least three times. *P < 0.05, **P < 0.01, ***P < 0.001, NS: no significance, by one‐way ANOVA.