Fig. 4.

Effects of the addition of FBS/BSA in collecting buffer on cell apoptosis. (A–C) Dot plots of apoptotic cells sorted with different collecting buffers. Numbers in quadrants indicate the percentages of cells in each throughout. Cells were stained with Annexin‐V and propidium iodide and analyzed by flow cytometry. A is Jurkat cells, B is 293T cells, and C is CD45⁺ cells from mouse spleen. (D–F) The concentration of FBS or BSA in the collection buffer affects the apoptotic rate. The data of the percentage of apoptotic cells shown in D–F are the means of three independent experiments. D is Jurkat cells, E is 293T cells, and F is CD45⁺ cells from mouse spleen. Data are presented as the mean ± SEM. n = 3. Each experiment was repeated at least three times. **P < 0.01, ***P < 0.001, NS: no significance, by one‐way ANOVA.