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. 2021 Aug 19;11(9):2607–2618. doi: 10.1002/2211-5463.13259

Fig. 2.

Fig. 2

Phenotypic changes of MDA‐MB‐231 cells in 3DM after TM treatment. (A) Light microscope images of MDA‐MB‐231 cells in 3DM and TM‐treated 3DM (3DM+TM). Scale bars: 200 μm. (B) F‐actin (red) and nuclear (blue) DAPI fluorescence images of MDA‐MB‐231 cells in 3DM and 3DM+TM. Scale bars: 100 μm. (C, D) Western blot analysis of VM marker protein VE‐cadherin and stem cell marker Bmi‐1 of MDA‐MB‐231 cells in 2D, 3DM and 3DM+TM. (E) Flow cytometric analysis of surface markers CD24 and CD44 in 3DM MDA‐MB‐231 cells. (F) Flow cytometric analysis of surface markers CD24 and CD44 in 3DM MDA‐MB‐231 cells after TM treatment.