Fig. 1.

Suppression of pulmonary infiltration of eosinophils by fasting. (A) Six‐week‐old C57BL/6 mice were inoculated 20 μL of PBS solution containing OVA (0.5 mg·mL⁻¹) and IL‐33 (5 μg·mL⁻¹) into noses on days 0 and 7. The mice were fasted for 48 h from day 0 to day 2. (B) The mice were fasted for 48 h from day 7 to day 9. (C) Gating strategy after the exclusion of doublets, dead fluorescent‐positive cells were shown. CD45⁺ Siglec‐F⁺ CD11c⁻ cells (eosinophils) and CD45⁺ Siglec‐F⁺ CD11c⁺ cells (alveolar macrophages) were analysed by flow cytometry. (D) Each column represents the total BAL cells (black), eosinophils (red) and alveolar macrophages (blue). Each value represents the mean ± S.E.M. of 9‐12 experiments. *P < 0.05 compared with each group. Statistical significance was determined by Scheffe’s test after performing an ANOVA test using StatView (SAS Institute, Inc., NC, USA).