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. 2021 Aug 20;11(9):2619–2630. doi: 10.1002/2211-5463.13268

Fig. 2.

Fig. 2

Effect of fasting on anti‐OVA total IgG1 and IgE production. (A) Six‐week‐old C57BL/6 mice were inoculated 20 μL of PBS solution containing OVA (0.5 mg·mL−1) and IL‐33 (5 μg·mL−1) into noses on days 0 and 7. The mice were fasted for 48 h from day 7 to day 9. And then, the mice were refed for 24 h from day 9 to day 10. BALF, serum, mLN and lung were collected on day 10. (B) Anti‐OVA total IgG1 and IgE in the serum were determined by ELISA. Each bar represents the mean of 9‐12 experiments. *P < 0.05 compared with each group. Statistical significance was determined by Scheffe’s test after performing an ANOVA test using StatView (SAS Institute, Inc., NC, USA). n.s.; not significant.