Fig. 5.

Effect of fasting on T cells in spleen and bone marrow. (A) The numbers of CD4⁺ T cell, memory CD4⁺ T cell, naïve CD4⁺ T cell and effector CD4⁺ T cell in the spleen were determined by flow cytometry. (B) The numbers of CD4⁺ T cell, memory CD4⁺ T cell, naïve CD4⁺ T cell and effector CD4⁺ T cell in the bone marrow were determined by flow cytometry. Each value represents the mean ± S.E.M. of 6–8 experiments. *P < 0.05 compared with each group. Statistical significance was determined by Scheffe’s test after performing an ANOVA test using StatView (SAS Institute, Inc., NC, USA). n.s.; not significant.