FIG. 2.

IR induces Rad51 cleavage by a CrmA-insensitive, p35-sensitive mechanism. U-937 cells stably expressing an empty vector, CrmA, or p35 were treated with 30 ng of TNF per ml for 6 h or with 20 Gy of IR and then harvested at 7 h. (A) DNA content was assessed by flow cytometry. (B) Cell lysates were subjected to immunoblotting analysis with anti-caspase 3, anti-PARP, or anti-Rad51.