FIGURE 1.

Expression of heparanase in glioma cells correlates with their resistance to temozolomide (TMZ). A, CCK‐8 assay was used to analyze the viability of glioma cells treated with different concentrations of TMZ (0, 0.1, 1, 5, 10, 20, 100, 200, 400 μM) for 48 hours, and the IC₅₀ of TMZ was calculated. B, The relationship between heparanase mRNA and the IC₅₀ of TMZ in glioma cells exposed to TMZ was analyzed. C, Viability of U251 and U251R cells exposed to TMZ was analyzed using CCK‐8 assay. CI, confidence interval. D, The expression of heparanase mRNA in U251R cells was markedly higher than that of U251 cells. E, Western blot assay was used to analyze the expression of heparanase in U251 and U251R cells. F, Proliferation of U251 and U251R cells was analyzed using CCK‐8 assay. *P < .05; **P < .01; ***P < .001