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. 2021 Jul 29;112(9):3437–3454. doi: 10.1111/cas.15033

FIGURE 1.

FIGURE 1

Melanoma‐released exosome is significantly increased as metastasis progression. To analysis the tumor‐released exosomes in metastasis, lung tissues were dissected out, digest, and then sorting melanoma cells for Ter119−CD31−CD45−CD146+ using flow cytometry. These cells were cultured in vitro for 24 h followed with exosome purification by gradient centrifugation. A, Electron microscopy images of purified exosomes. B, ELISA to measure the levels of exosome isolated from mice (n = 5) on days 0, 3, 6, 9, 12 15, or 18. The levels shown are ng per 100 mg weight of lung tissue. C, Pearson correlation between the exosome level and tumor burden (number of metastases foci) in mice (n = 20). D, Sorted melanoma cells were treated with or without IFN‐γ, and then the levels of exosome were measured by ELISA on different days. In (B) and (D), the experiments were performed at least twice with similar results. Error bars, SEM