FIG. 1.

Yor1p localization by indirect immunofluorescence. Isogenic strains with various levels of the YOR1 gene were prepared for indirect immunofluorescence essentially as described previously (67). Strains carried the yor1-1::hisG allele (left panels), had a single copy of YOR1 (middle panels), or were transformed with the wild-type YOR1 gene carried on a 2 μm plasmid (right panels). Cells were labeled with affinity-purified rabbit anti-Yor1p C-terminal antibody, followed by incubation with fluorescein isothiocyanate-conjugated goat anti-rabbit antibody.