FIG. 4.

Oligomycin resistance phenotypes of Yor1p and mutant variants. DKY7 (yor1-1::hisG) cells were transformed with low-copy-number plasmids bearing the genes that express the indicated forms of Yor1p or with the low-copy-number vector (pRS316). Transformants were grown to an A₆₀₀ of approximately 1, and 1,000 cells were placed on YPGE medium containing a gradient of oligomycin (indicated by the wedge of increasing height). The plate was incubated at 30°C and photographed.