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Steady-state levels of mutant Yor1p derivatives. Whole-cell extracts were prepared from DKY7 (yor1-1::hisG) cells expressing the indicated forms of Yor1p from low-copy-number plasmids. Protein (75 μg) was electrophoresed by SDS-PAGE, transferred to nitrocellulose, and blotted with anti-Yor1p (A) or anti-Vph1p (B) as a loading control.
