FIG. 6.

Subcellular fractionation of Yor1p NBD1 mutants. Cells lacking a chromosomal YOR1 locus (DKY7) were transformed with low-copy-number plasmids bearing the genes that express the indicated forms of Yor1p. Lysates were prepared and centrifuged through sucrose gradients as described in the legend to Fig. 2. Aliquots of each sucrose gradient were then subjected to Western blotting with the affinity-purified anti-Yor1p antibody (Yor1p) or the rabbit anti-Pma1p (Pma1p), rabbit anti-Vps10p (Vps10p), or rabbit anti-Sec61p (Sec61p) antiserum as listed on the right-hand side of each panel. The orientation of the sucrose gradient fractions is indicated as in Fig. 2.