FIG. 7.

Vacuolar proteases influence the turnover of both wild-type and ΔF670 Yor1p. Isogenic yor1-1::hisG strains that either contained (top panels) or lacked (bottom panels) the PEP4 locus were transformed with low-copy-number plasmids bearing the gene that expresses the wild-type or ΔF670 form of Yor1p. Selected transformants were then analyzed by pulse-chase analysis followed by immunoprecipitation with the anti-Yor1p antiserum or an anti-CPY polyclonal antibody (from R. Piper). Levels of immunoprecipitated proteins were quantitated by phosphorimaging. The time scale for the pulse-chase in the PEP4 background was in minutes, while that for the pulse-chase with the pep4 strain was in hours. The position of Yor1p and the positions of the various forms of CPY (58) are denoted by arrows.