FIG. 8.

Mg²⁺-dependent fractionation of ΔF670 Yor1p in PEP4 or pep4 cells. Lysates were prepared from PEP4 (A) or pep4 (B) cells expressing either wild-type or ΔF670 Yor1p. These lysates were subjected to sucrose gradient centrifugation in the presence or absence of Mg²⁺ as described previously (53). Aliquots of the gradient were then analyzed by Western blotting (see Materials and Methods) for the presence of either form of Yor1p and for Sec61p or Vph1p.