FIG. 9.

Effect of loss of Sec12p function on turnover of wild-type and ΔF670 Yor1p. A strain containing the temperature-sensitive sec12-3 allele and lacking the YOR1 gene was constructed by one-step gene disruption of the YOR1 locus to produce EAE18. Low-copy-number plasmids bearing the gene that expresses either the wild-type or ΔF670 form of Yor1p were then introduced into this gene background. Turnover of these two forms of Yor1p was then assessed by pulse-chase immunoprecipitation analysis at the permissive (23°C) and restrictive (37°C) temperatures. The numbers refer to times in minutes after chase addition.