FIGURE 1.

Establishment of stable transfectants expressing the fluorescent ubiquitination‐based cell cycle indicator (Fucci) reporter system. A‐D, Stable transfectants expressing the Fucci reporter system (A549‐Fucci cells [A, B] and 4T1‐Fucci cells [C, D]) were treated with or without transforming growth factor‐β1 (TGF‐β1; 5 ng/mL) for 1 d. The morphologies, DAPI staining, Fucci patterns, and 5‐ethynyl‐2′‐deoxyuridine (EdU) labeling of the cells are shown in (A) and (C). Cell cycle was analyzed by flow cytometry and shown in (B) and (D). hCdt1, human Cdt1; hGem, human Geminin; mAG, monomeric Azami Green