FIGURE 4.

Effects of antitumor drugs on 4T1‐fluorescent ubiquitination‐based cell cycle indicator (Fucci) cells in vitro. A, Effect of antitumor drugs on 4T1‐Fucci cells. 4T1‐Fucci cells were treated with 5‐fluorouracil (5‐FU), cisplatin (CDDP), and paclitaxel (PTX) at the indicated concentrations for 2 d. Cell proliferation was evaluated by WST‐8 assay. The experiment was repeated three times and representative results are shown (average ± SD). B, C, Effect of antitumor drugs on cell cycle of 4T1‐Fucci cells. 4T1‐Fucci cells were treated with antitumor drugs at low concentrations for 24 h. Cell images are shown in (B). Enlarged images of white insets of (B) are shown in (C). Fragmented nuclear divisions are indicated by white arrowheads in (C). Ctrl, control; hCdt1, human Cdt1; hGem, human Geminin; mAG, monomeric Azami Green