FIGURE 7.

Effects of 5‐fluorouracil (5‐FU) on 4T1‐fluorescent ubiquitination‐based cell cycle indicator (Fucci) tumors in vivo. A, Effects of 5‐FU on 4T1‐Fucci tumors. 4T1‐Fucci cells were inoculated intravenously (day 0). On day 3 to day 9, mice were treated with 5‐FU (35 mg/kg) or control saline (n = 6 per group). On day 10, mice were killed and lungs were excised and subjected to clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC) procedures. Representative 3D and 2D images of lungs are shown. B, C, Transparent samples in (A) were washed with PBS and embedded in paraffin blocks. H&E staining and immunohistochemistry with anti‐Ki‐67 Ab was carried out. Representative images of the samples in saline and 5‐FU are shown; (B) H&E, (C) Ki‐67 staining. hCdt1, human Cdt1; hGem, human Geminin; mAG, monomeric Azami Green