FIGURE 3.

Inhibition of pyruvate dehydrogenase kinase 4 (PDK4) by shRNA or dichloroacetate (DCA) sensitizes diffuse large B‐cell lymphoma (DLBCL) cells to rituximab (RTX). A, B, Annexin V‐phycoerythrin (PE)/7‐AAD double staining analysis of DLBCL cells treated with RTX (50 μg/mL). Interference with PDK4 shRNA increased RTX‐induced cell apoptosis and caspase‐3 activation in U2932 and OCI‐Ly8 cell lines. C, PDK4 inhibitor dichloroacetate (DCA) enhanced the RTX‐induced apoptosis in DLBCL cell lines U2932, OCI‐ly7, and OCI‐Ly8. cop‐GFP, copepod super green fluorescent protein. *P < .05, **P < .01