FIGURE 6.

Pyruvate dehydrogenase kinase 4 (PDK4) mediates the metabolic shift of rituximab‐resistant diffuse large B‐cell lymphoma cells. A‐D, U2932 and OCI‐ly8 cells were transfected with cop‐GFP or interfered PDK4 shRNA (PDK4‐sh1) and the glucose consumption (A), lactate production (B), ATP levels (C), and cellular extracellular acidification rate (ECAR) (D) were measured. E, OCI‐Ly8 cells were transfected with PDK4 overexpressing (PDK4 OE) plasmid or empty vector (EV). After treatment with or without dichloroacetate (DCA, 5 mM), cellular ECAR were measured. cop‐GFP, copepod super green fluorescent protein. *P < .05, **P < .01