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. 2021 Jun 29;112(9):3722–3731. doi: 10.1111/cas.15023

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© 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Carcinoembryonic antigen 5 (CEA) is a glycoprotein ligand of rBC2LCN in a pancreatic cancer cell line. A, Immunofluorescence staining of rBC2LCN (green), CEA (red), and nuclei (blue) in Capan‐1 and SUIT‐2 cells. Merge: yellow. Original magnification: ×1000. Scale bars, 50 μm. B, Flow cytometry analysis of CEA and rBC2LCN ligands in Capan‐1 and SUIT‐2 cells. C, Lectin and Western blotting of cell lysates for rBC2LCN and CEA and silver staining of cell lysate. D, rBC2LCN‐precipitated fractions were blotted with rBC2LCN or anti‐CEA polyclonal antibody (pAb) (αCEA) and silver stained