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. 2021 Jun 29;112(9):3722–3731. doi: 10.1111/cas.15023

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© 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Carcinoembryonic antigen 5 (CEA) is a glycoprotein ligand of rBC2LCN in clinical pancreatic ductal adenocarcinoma (PDAC) samples. A, Representative immunofluorescence staining of rBC2LCN (green) and CEA (red) in clinical PDAC tissues. Nuclei: blue. Merge: yellow. Scale bars, 100 μm. B, Lectin blot and Western blot analysis of rBC2LCN and CEA in PDAC tissues (n = 6) and normal pancreatic tissues (n = 3) and silver staining. C, rBC2LCN‐precipitated fractions were blotted with anti‐CEA polyclonal antibody (pAb) and silver stained. D, αCEA‐precipitated fractions were blotted with rBC2LCN and silver stained