FIGURE 3.

Co‐immunization with L‐Myc enhances the induction and mature of DC subsets. Mice immunized with vaccines were sacrificed on the 42 days post tumor inoculation, the proportions of DCs subsets were analyzed by flow cytometry in spleen. (A) The representative flow cytometry of CD11c⁺, CD103⁺CD11c⁺ or CD8⁺CD11c⁺ DCs subpopulation. (B‐D) Statistical analysis of the percentages of DCs subsets in (A). (E) The expression of CD80, CD86, or MHC‐II on CD11c⁺ cells. (F‐H) Statistical analysis of the frequencies of CD11c⁺CD80⁺, CD11c⁺CD86⁺ or CD11c⁺MHC II⁺ cells in (E). The data shown are the representative of three experiments. Data are means ±SD. The different significance was set at**P <.01, ***P <.001