FIGURE 5.

CS‐pL‐Myc/pCAIX co‐immunization increased the induction of multi‐functional CD8⁺ T cells. 42 days after the tumor inoculation, splenocytes or TILs from vaccine immunized mice were stimulated in vitro. (A and B) The proportions of t TNF‐α⁺IL‐2⁺CD8⁺ T cells in spleen or TILs were detected by flow cytometry. (C and D) The percentages of TNF‐α⁺IFN‐γ⁺CD8⁺ T cells. (E and F) The percentages of IL‐2⁺IFN‐γ⁺CD8⁺ T cells. (G and H) The proportions of TNF‐α⁺IL‐2⁺IFN‐γ⁺CD8⁺ T cells. The data shown are the representative of three experiments. Data are means ±SD. The different significance was set at **P <.01, ***P <.001