FIGURE 7.

CS‐pL‐Myc/pCAIX co‐immunization suppressed the lung metastasis in renal carcinoma model by enhancing multi‐functional CD8⁺ T cell responses. (A) Schema of lung metastasis model establishment and experiment. (B) The present images of lung metastasis tumors excised from mice. (C) The numbers of metastatic nodules. (D) Lung tissues performed by H&E staining. (E) CD8⁺ T cells of lung tissues were detected by immunochemistry staining (×200 magnification). (F) The frequencies of CD8⁺ T cells were analyzed by flow cytometry in the lung tumor tissues. (G) Intracellular staining of TNF‐α, IFN‐γ, and IL‐2 of multi‐functional CD8⁺ T cells in stimulated splenocytes. (H) CAIX‐specific IFN‐γ‐secreting T lymphocyte cells were quantified by ELISPOT assay. (I) The CTL was detected by RTCA assay. (J) Cell index by RTCA assay were analyzed in (I). The experiments were performed with five mice per group. Data are from one representative experiment of three performed and presented as the mean ±SD. The different significance was set at *P <.05, **P <.01, ***P <.001