FIGURE 1.

Identification of the 5'‐regulatory region of HNRNPLL. A, The gene structure of HNRNPLL. Boxes: exons; dark boxes, coding region; light boxes, non‐coding region; arrow, gene‐specific primer site for 5'‐RACE analysis. Two variants for the first exon are shown with RefSeq accession numbers. B, Electrophoresis of the 5'‐RACE PCR product. Arrow and arrowhead show bands corresponding to NM_138394 and NM_001142650, respectively. C, Schema of the reporter constructs. D, Luciferase activity of the R1‐5 reporters measured in HT29 and DLD‐1 cells. *P < .001; NS, not significant (P > .05). E, Nucleic acid sequence including the 5'‐regulatory region of HNRNPLL (−273 to +46). Locations of the individual forward primers used for the reporter constructs and their common reverse primer (Rev) are indicated with arrows. (The R6‐9 reporter constructs are used in Figures 2 and 3) The transcription start site is indicated with shaded "G". Potential binding sites for MYB and SP1 are shown with bold and double underlines, respectively