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. 2021 Jul 29;112(9):3846–3855. doi: 10.1111/cas.15069

FIGURE 2.

FIGURE 2

The MYB family proteins are involved in the transcriptional regulation of HNRNPLL. A, Western blot analysis for expression of MYB, MYBL1, and MYBL2 in HT29 and DLD‐1 cells with or without EMT‐induction. B, Reporter constructs for addressing the two potential MYB‐binding sites. Filled squares indicate additional reporters prepared for experiments in Figure 2. R4‐M1 and R7‐M2 are mutant constructs with synthetic base substitution in the distal and proximal MYB sites, respectively. C, Luciferase activity of the reporter constructs in HT29 and DLD‐1 cells. *P < .05; **P < .01; NS, not significant (P > .05). D, Quantitative RT‐PCR analysis for expression of HNRNPLL in HT29 and DLD‐1 cells transduced with CRISPR‐Cas9 for MYB and/or MYBL2. *P < .001; NS, not significant (P > .05). E, Luciferase activity of the reporter constructs in MYB‐knocked out cells. *P < .05; **P < .01; NS, not significant (P > .05). F, Agarose gel electrophoresis of the PCR products amplified from chromatins immunoprecipitated from FLAG‐MYB‐introduced cells using the ChIP primers F1 and R1. G, Quantitative RT‐PCR analysis for expression levels of HNRNPLL in EMT‐induced cells with or without MYB‐overexpression. *P < .0005; **P < .01; NS, not significant (P > .05)