FIGURE 3.

SP1 and SP3 are involved in the transcriptional regulation of HNRNPLL. A, Reporter constructs for addressing the potential SP1‐binding sites. Filled squares indicate reporters additionally prepared for experiments in Figure 3. B, Luciferase activity of the reporter constructs in HT29 and DLD‐1 cells. The effect of the SP1/3 inhibitor mithramycin A (MiA) was also tested for R6. *P < .001; NS, not significant (P > .05). C, Quantitative RT‐PCR analysis for expression levels of HNRNPLL in HT29 and DLD‐1 cells transduced with CRISPR‐Cas9 for SP1 and/or SP3. *P < .01; **P < .001; ^† P < .05. D, Agarose gel electrophoresis of the PCR products amplified from chromatins immunoprecipitated from FLAG‐SP1‐ or FLAG‐SP3‐overexpressed cells. E, Western blot analysis for expression levels of SP1 and SP3 in HT29 and DLD‐1 cells with or without EMT induction