Fig. 4. In vivo decarboxylation. (a, b) Quantification of levels of F-5caC and F-dC in genomic DNA upon feeding of F-5caC with diverse concentrations to HEK293T cells. (c) Quantification of the levels of F-5caC and F-dC in genomic DNA upon feeding of 300 μM of F-5caC to wt or TDG−/− HEK293T cells. (d) Peak area ratio of F-dC/F-5caC in F-5caC (300 μM) metabolically labeled genomic DNA upon incubation with the recombinant human TDG protein or buffer.
