Figure 2. Inactivation of ANGPTL4 in hepatocytes enhances plasma TAG clearance and hepatic lipid uptake.

(A and B) Oral lipid tolerance test showing the clearance of TAG and NEFA from the plasma of WT and Hmut mice fasted for 4 hours, followed by oral gavage of olive oil. Inset represents the AUC. (C) Radioactivity incorporation in indicated tissues after 2 hours of oral gavage of [³H]-labeled triolein in WT or Hmut mice fasted for 4 hours. Inset represents plasma lipid clearance. (D and E) HL and LPL activity in the postheparin plasma from overnight-fasted WT and Hmut mice. (F) Plasma TAG from overnight-fasted WT and Hmut mice treated with plasma LPL inhibitor poloxamer 407 to inhibit the hydrolysis of circulating TAG (n = 5). (G) Serum ³H-labled triolein counts after injection of poloxamer 407 combined with oral lipid gavage containing ³H-labeled triolein (n = 5). All data are represented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, comparing Hmut with WT mice using the unpaired t test.