Skip to main content
. 2021 Sep 1;131(17):e145472. doi: 10.1172/JCI145472

Figure 2. NOD2 stimulation induces an RNF186 complex with UPR sensors.

Figure 2

(A) MDMs were treated with 100 μg/mL MDP for the indicated times. ER and cytosolic fractions were assessed for RIP2 by Western blot. Markers for ER (calnexin) and cytosolic (GAPDH) fractions are shown. Representative of 2 independent experiments. (B) MDMs were treated with 100 μg/mL MDP for 30 minutes. ATF6 was immunoprecipitated followed by immunoblotting (IB) of the indicated proteins. Representative of 2 independent experiments. (C) MDMs were transfected with scrambled or RNF186 siRNA and then treated with 100 μg/mL MDP for 30 minutes. RIP2 or ATF6 was immunoprecipitated, and recruitment of the indicated proteins was assessed by IB. Representative of 2–3 independent experiments. Expression of the respective proteins and GAPDH in whole-cell lysates (WCLs) served as loading controls. Marker positions are shown (kDa). Scr, scrambled.